Determination of severity and prognosis of acute pancreatitis using electron and atomic force microscopy
G. N. Gulikyan,
R. A. Pakhomova,
S. I. Petrushko,
Yu. M. Volkov,
N. M. Markelova,
L. V. Kochetova,
Yu. A. Nazariants,
L. I. Shvets https://doi.org/10.17238/2072-3180-2025-4-26-37
Abstract
Introduction. The purpose of this study is to determine the predictors of the severity of acute pancreatitis by electron and atomic force microscopy of peripheral blood erythrocytes.
Materials and methods. Electron and atomic force microscopy of peripheral blood erythrocytes of healthy people and patients with pancreatitis of varying severity was performed. Venous blood sampling was performed from the cubital vein upon admission to the hospital. Blood from donors was used as a control group. The shape of the erythrocyte, the volume of the erythrocyte, the state of the membrane and cytoplasm, as well as the content of potassium, sodium, calcium, nitrogen and oxygen in the cell were compared.
Results. Electron and atomic force microscopy of red blood cells revealed a change in the size and membrane of red blood cells, significantly dependent on the severity of acute pancreatitis, as well as the effect of membrane-protective therapy on the condition of red blood cells. Discussion. Comparative scanning of erythrocyte membranes can clearly trace the negative changes in the latter compared to the membrane of healthy people and depending on the severity of the process.
Conclusions. According to these changes in red blood cells, it is possible to indirectly judge the processes occurring in the cells of the pancreas. Accordingly, it is possible to determine the severity of acute pancreatitis.
About the Authors
G. N. Gulikyan
HUZ "Clinical hospital "Russian Railways-Medicine" of the city of Krasnoyarsk"
Russian Federation
Russian Federation
Gulikyan Garen Norayrovich – PhD, Associate Professor of Plastic Surgery at Rosbiotech.
47 Lomonosova St., Krasnoyarsk, 660058
R. A. Pakhomova
Rosbiotech Federal State Budgetary Educational Institution
Russian Federation
Russian Federation
Pakhomova Regina Alexandrovna – Professor, MD, Head of the Department of Plastic Surgery at Rosbiotech.
11, Volokolamsk Highway, Moscow, 125080
S. I. Petrushko
Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky
Russian Federation
Russian Federation
Petrushko Stanislav Ivanovich – Head of the Department of General Surgery named after Professor M.I. Gulman
Partizan Zheleznyak str., 1, Krasnoyarsk, 660022
Yu. M. Volkov
Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky
Russian Federation
Russian Federation
Volkov Yuri Mikhailovich – MD, Professor of the Department of General Surgery named after Prof. M.I. Gulman
Partizan Zheleznyak str., 1, Krasnoyarsk, 660022
N. M. Markelova
Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky
Russian Federation
Russian Federation
Markelova Nadezhda Mikhailovna – Professor of the M.I. Gulman Department of General Surgery
Partizan Zheleznyak str., 1, Krasnoyarsk, 660022
L. V. Kochetova
Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky
Russian Federation
Russian Federation
Kochetova Lyudmila Victorovna – Candidate of Medical Sciences, Associate Professor; Professor of the Department of General Surgery named after Professor M.I. Gulman
Partizan Zheleznyak str., 1, Krasnoyarsk, 660022
Yu. A. Nazariants
Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky
Russian Federation
Russian Federation
Nazariants Yulia Andreevna – Associate Professor of the Department of General Surgery named after Professor M.I. Gulman
Partizan Zheleznyak str., 1, Krasnoyarsk, 660022
L. I. Shvets
MK RUT(MIIT)
Russian Federation
Russian Federation
Shvets Lyubov Igorevna – a teacher of the highest category
129128, Moscow, Budaiskaya str., 2, building 18
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Review
For citations:
Gulikyan G.N., Pakhomova R.A., Petrushko S.I., Volkov Yu.M., Markelova N.M., Kochetova L.V., Nazariants Yu.A., Shvets L.I. Determination of severity and prognosis of acute pancreatitis using electron and atomic force microscopy. Moscow Surgical Journal. 2025;(4):26-35. (In Russ.) https://doi.org/10.17238/2072-3180-2025-4-26-37
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